Optical Injection and Manipulation of Functional Naotool Using Photo-responsive Chemical and Opticaltweezers for Intracellulat Measurement
نویسندگان
چکیده
We developed photo-induced selective injection of nanotool into a specific cell for intracellular measurement in a microfluidic chip. We used membrane fusion of the liposome for invasive cell injection of the nanotool. The nanotool was modified by photo-responsive chemical for pH control and fluorescent dye for environment measurement. The nanotool was included in fusogenic liposome. Fusion-capability of the liposome was switched by optical pH control. The liposome was transported by optical tweezers to the cell surface. The liposome was fused to the cell and the nanotool was injected to the cell invasively by optical pH control. Injected nanotool was manipulated by optical tweezers and intracellular environment was measured by fluorescence observation.
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